Journal: Journal of Nanobiotechnology

Article Title: Neutrophil membrane-coated and MMP2-responsive nanoparticles deliver PIM1 inhibitor to alleviate inflammatory arthritis through inhibiting Th17 cell differentiation

doi: 10.1186/s12951-026-04280-x

Figure Lengend Snippet: Biological characteristics of NM@MRP-NP. ( A ) Western blot analysis of the expression of CD11a, CD11b and L-selectin on the neutrophil membrane before and after LPS treatment ( n = 9). ( B ) Representative immunofluorescence images of CD11a, CD11b and L-selectin on the neutrophil membrane before and after LPS treatment. Scale bar = 20 μm. ( C - D ) Western blot analysis ( C ) and immunofluorescence staining ( D ) confirmed the transfer of CD11a, CD11b and L-selectin to the NM@MRP-NP. Scale bar = 100 μm. ( E - F ) Uptake of Cy5.5 by activated or unactivated CD4 + T cells treated with vehicle, MRP-NP, NM@MRP-NP or NM@MRP-NP+MMP2 ( n = 9). The statistical analyses were performed as follows: paired t test (A, F) and one-way ANOVA followed by Bonferroni’s post hoc comparisons test ( F ). ** indicates p < 0.01, *** indicates p < 0.001, **** indicates p < 0.0001, and n.s. indicates no statistical significance

Article Snippet: The antibodies used were as follows: CD11a (ABclonal, # A23960 ), CD11b (ABclonal, #A26754PM), L-selectin (ABclonal, #A1622), Na, K-ATPase (Cell Signaling Technology, #3010), β-Actin (Cell Signaling Technology, #3700), RORγt (Abcam, #ab113434), STAT3 (Abcam, #ab68153), phospho-STAT3 (Abcam, #ab76315), HRP-conjugated goat anti-mouse IgG (H + L) (Boster, #BA1051) and HRP-conjugated goat anti-rabbit IgG (H + L) (Boster, #BA1055).

Techniques: Western Blot, Expressing, Membrane, Immunofluorescence, Staining